› 2vp8

X-ray diffraction
2.64Å resolution

Structure of Mycobacterium tuberculosis Rv1207

Released:
DOI: 10.2210/pdb2vp8/pdb
PDB entry 2vp8 coloured by chain, front view.
PDB entry 2vp8 coloured by chain, side view.
PDB entry 2vp8 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Biochemical and structural characterization of the putative dihydropteroate synthase ortholog Rv1207 of Mycobacterium tuberculosis.
Gengenbacher M, Xu T, Niyomrattanakit P, Spraggon G, Dick T
FEMS Microbiol Lett 287 128-35 (2008)
PMID: 18680522

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161850 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inactive dihydropteroate synthase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 318 amino acids
Theoretical weight: 33.05 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WNC9 (Residues: 1-318; Coverage: 100%)
Gene names: MTCI364.19, Rv1207, folP2
Sequence domains: Pterin binding enzyme
Structure domains: Dihydropteroate synthase-like

Ligands and Environments

1 bound ligand:
Ligand EDO 4 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: P3221
Unit cell:
a: 80.727Å b: 80.727Å c: 215.938Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.226 0.223 0.268
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Mycobacterium tuberculosis folate metabolism and the mechanistic basis for para-aminosalicylic acid susceptibility and resistance.
Minato et al. (2015)
2 more

1 mention without citation

A unified statistical model to support local sequence order independent similarity searching for ligand-binding sites and its application to genome-based drug discovery.
Xie et al. (2009)