› 2vpj

X-ray diffraction
1.85Å resolution

Crystal structure of the Kelch domain of human KLHL12

Released:
DOI: 10.2210/pdb2vpj/pdb
PDB entry 2vpj coloured by chain, front view.
PDB entry 2vpj coloured by chain, side view.
PDB entry 2vpj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for Cul3 protein assembly with the BTB-Kelch family of E3 ubiquitin ligases.
Canning P, Cooper CDO, Krojer T, Murray JW, Pike ACW, Chaikuad A, Keates T, Thangaratnarajah C, Hojzan V, Marsden BD, Gileadi O, Knapp S, von Delft F, Bullock AN
J Biol Chem 288 7803-7814 (2013)
PMID: 23349464

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176096 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Kelch-like protein 12 Chain: A
Molecule details ›
Chain: A
Length: 301 amino acids
Theoretical weight: 32.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q53G59 (Residues: 267-567; Coverage: 53%)
Gene names: C3IP1, KLHL12
Sequence domains: Kelch motif
Structure domains: Kelch-type beta propeller

Ligands and Environments

1 bound ligand:
Ligand ACT 2 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 44.637Å b: 61.546Å c: 45.523Å
α: 90° β: 111.75° γ: 90°
R-values:
R R work R free
0.164 0.161 0.222
Expression system: Escherichia coli BL21(DE3)

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Citations

45 review citations

The KEAP1-NRF2 System: a Thiol-Based Sensor-Effector Apparatus for Maintaining Redox Homeostasis.
Yamamoto et al. (2018)
44 more

12 mentions without citation

Drug resistance genomics of the antimalarial drug artemisinin.
Winzeler et al. (2014)
11 more