› 2vpr

X-ray diffraction
2.49Å resolution

Tet repressor class H in complex with 5a,6- anhydrotetracycline-Mg

Released:
DOI: 10.2210/pdb2vpr/pdb
PDB entry 2vpr coloured by chain, front view.
PDB entry 2vpr coloured by chain, side view.
PDB entry 2vpr coloured by chain, top view.
Source organism: Pasteurella multocida
Primary publication:
Tet repressor induction by tetracycline: a molecular dynamics, continuum electrostatics, and crystallographic study.
Aleksandrov A, Schuldt L, Hinrichs W, Simonson T
J Mol Biol 378 898-912 (2008)
PMID: 18395746

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156359 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tetracycline repressor protein class H Chain: A
Molecule details ›
Chain: A
Length: 207 amino acids
Theoretical weight: 23.21 KDa
Source organism: Pasteurella multocida
Expression system: Escherichia coli
UniProt:
  • Canonical: P51561 (Residues: 1-207; Coverage: 100%)
Gene name: tetR
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand TDC 1 x TDC
Ligand MG 1 x MG
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X13
Spacegroup: C2221
Unit cell:
a: 56.361Å b: 108.744Å c: 68.976Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.201 0.285
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Transcription factor-based biosensors enlightened by the analyte.
Fernandez-López et al. (2015)
17 other articles