› 2vrb

X-ray diffraction
2Å resolution

Crystal structure of the Citrobacter sp. triphenylmethane reductase complexed with NADP(H)

Released:
DOI: 10.2210/pdb2vrb/pdb
PDB entry 2vrb coloured by chain, front view.
PDB entry 2vrb coloured by chain, side view.
PDB entry 2vrb coloured by chain, top view.
Source organism: Citrobacter sp. MY-5
Primary publication:
Structural insight into bioremediation of triphenylmethane dyes by Citrobacter sp. triphenylmethane reductase.
Kim MH, Kim Y, Park HJ, Lee JS, Kwak SN, Jung WH, Lee SG, Kim D, Lee YC, Oh TK
J Biol Chem 283 31981-90 (2008)
PMID: 18782772

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174052 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NmrA-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 287 amino acids
Theoretical weight: 30.98 KDa
Source organism: Citrobacter sp. MY-5
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2TNI4 (Residues: 1-287; Coverage: 100%)
Sequence domains: NmrA-like family
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand NAP 1 x NAP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 4A
Spacegroup: C2221
Unit cell:
a: 58.8Å b: 141.645Å c: 75.811Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.2 0.242
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Microbial Degradation of Azo Dyes: Approaches and Prospects for a Hazard-Free Conversion by Microorganisms.
Ngo et al. (2022)
13 other articles

1 mention without citation

Substrate-Assisted Catalysis in Polyketide Reduction Proceeds via a Phenolate Intermediate.
Schäfer et al. (2016)