› 2vrq

X-ray diffraction
2Å resolution

STRUCTURE OF AN INACTIVE MUTANT OF ARABINOFURANOSIDASE FROM THERMOBACILLUS XYLANILYTICUS IN COMPLEX WITH A PENTASACCHARIDE

Released:
DOI: 10.2210/pdb2vrq/pdb
PDB entry 2vrq coloured by chain, front view.
PDB entry 2vrq coloured by chain, side view.
PDB entry 2vrq coloured by chain, top view.
Source organism: Thermobacillus xylanilyticus
Primary publication:
The structure of the complex between a branched pentasaccharide and Thermobacillus xylanilyticus GH-51 arabinofuranosidase reveals xylan-binding determinants and induced fit.
Paës G, Skov LK, O'Donohue MJ, Rémond C, Kastrup JS, Gajhede M, Mirza O
Biochemistry 47 7441-51 (2008)
PMID: 18563919

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130649 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Alpha-L-arabinofuranosidase C-terminal domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 496 amino acids
Theoretical weight: 56.18 KDa
Source organism: Thermobacillus xylanilyticus
Expression system: Escherichia coli
UniProt:
  • Canonical: O69262 (Residues: 1-496; Coverage: 100%)
Gene name: AbjA
Sequence domains: Alpha-L-arabinofuranosidase C-terminal domain
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: XYP, AHR
Carbohydrate polymer
Carbohydrate polymer : NEW Components: XYP, AHR
Carbohydrate polymer
1 bound ligand:
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P6522
Unit cell:
a: 156.8Å b: 156.8Å c: 378.6Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.182 0.182 0.201
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Biotransformation of ginsenosides by hydrolyzing the sugar moieties of ginsenosides using microbial glycosidases.
Park et al. (2010)
3 more

12 mentions without citation

The Role of Arabinogalactan Type II Degradation in Plant-Microbe Interactions.
Villa-Rivera et al. (2021)
11 more