› 2vus

X-ray diffraction
2.6Å resolution

Crystal structure of unliganded NmrA-AreA zinc finger complex

Released:
DOI: 10.2210/pdb2vus/pdb
PDB entry 2vus coloured by chain, front view.
PDB entry 2vus coloured by chain, side view.
PDB entry 2vus coloured by chain, top view.
Source organism: Aspergillus nidulans
Primary publication:
Structural analysis of the recognition of the negative regulator NmrA and DNA by the zinc finger from the GATA-type transcription factor AreA.
Kotaka M, Johnson C, Lamb HK, Hawkins AR, Ren J, Stammers DK
J Mol Biol 381 373-82 (2008)
PMID: 18602114

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-148066 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Nitrogen metabolite repression protein nmrA Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 352 amino acids
Theoretical weight: 38.84 KDa
Source organism: Aspergillus nidulans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5AU62 (Residues: 1-352; Coverage: 100%)
Gene names: AN8168, nmrA
Sequence domains: NmrA-like family
Structure domains:
Nitrogen regulatory protein areA Chains: I, J, K, L, M, N, O, P
Molecule details ›
Chains: I, J, K, L, M, N, O, P
Length: 43 amino acids
Theoretical weight: 4.79 KDa
Source organism: Aspergillus nidulans
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P17429 (Residues: 670-712; Coverage: 5%)
Gene names: AN8667, areA
Sequence domains: GATA zinc finger
Structure domains: Erythroid Transcription Factor GATA-1, subunit A

Ligands and Environments

3 bound ligands:
Ligand SO4 8 x SO4
Ligand CL 7 x CL
Ligand ZN 8 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: R3
Unit cell:
a: 228.788Å b: 228.788Å c: 222.296Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.242 0.242 0.306
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli BL21

Quick links

Citations

2 review citations

Survey of the year 2008: applications of isothermal titration calorimetry.
Falconer et al. (2010)
1 more

1 mention without citation

An incoherent feedforward loop facilitates adaptive tuning of gene expression.
Hong et al. (2018)