› 2vuw

X-ray diffraction
1.8Å resolution

Structure of human haspin kinase domain

Released:
DOI: 10.2210/pdb2vuw/pdb
PDB entry 2vuw coloured by chain, front view.
PDB entry 2vuw coloured by chain, side view.
PDB entry 2vuw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure and functional characterization of the atypical human kinase haspin.
Eswaran J, Patnaik D, Filippakopoulos P, Wang F, Stein RL, Murray JW, Higgins JM, Knapp S
Proc Natl Acad Sci U S A 106 20198-203 (2009)
PMID: 19918057

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186268 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase haspin Chain: A
Molecule details ›
Chain: A
Length: 336 amino acids
Theoretical weight: 38.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8TF76 (Residues: 465-798; Coverage: 42%)
Gene names: GSG2, HASPIN
Sequence domains: Haspin like kinase domain
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand IOD 1 x IOD
Ligand 5ID 1 x 5ID
Ligand NI 1 x NI
Ligand DMS 1 x DMS
Ligand MPD 1 x MPD
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 78.661Å b: 78.775Å c: 79.86Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.148 0.147 0.169
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

19 review citations

The structural basis for control of eukaryotic protein kinases.
Endicott et al. (2012)
18 more

7 mentions without citation

On the molecular mechanisms of mitotic kinase activation.
Bayliss et al. (2012)
6 more