› 2vvj

X-ray diffraction
2Å resolution

IrisFP fluorescent protein in its red form, cis conformation

Released:
DOI: 10.2210/pdb2vvj/pdb
Model geometry
Fit model/data
PDB entry 2vvj coloured by chain, front view.
PDB entry 2vvj coloured by chain, side view.
PDB entry 2vvj coloured by chain, top view.
Source organism: Lobophyllia hemprichii
Primary publication:
Structural characterization of IrisFP, an optical highlighter undergoing multiple photo-induced transformations.
Adam V, Lelimousin M, Boehme S, Desfonds G, Nienhaus K, Field MJ, Wiedenmann J, McSweeney S, Nienhaus GU, Bourgeois D
Proc Natl Acad Sci U S A 105 18343-8 (2008)
PMID: 19017808

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177809 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Green to red photoconvertible GFP-like protein EosFP Chains: aa, bb, cc, dd
Molecule details ›
Chains: aa, bb, cc, dd
Length: 63 amino acids
Theoretical weight: 6.93 KDa
Source organism: Lobophyllia hemprichii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5S6Z9 (Residues: 1-61; Coverage: 27%)
Green to red photoconvertible GFP-like protein EosFP Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 163 amino acids
Theoretical weight: 19.06 KDa
Source organism: Lobophyllia hemprichii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5S6Z9 (Residues: 65-226; Coverage: 72%)
Sequence domains: Green fluorescent protein

Ligands and Environments

2 bound ligands:
Ligand SO4 15 x SO4
Ligand SO3 4 x SO3
2 modified residues:
Ligand NFA 4 x NFA
Ligand RC7 4 x RC7

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 85.67Å b: 96.62Å c: 140.17Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.186 0.232
Expression system: Escherichia coli

Quick links

Citations

46 review citations

Fluorescent proteins and their applications in imaging living cells and tissues.
Chudakov et al. (2010)
45 more

2 mentions without citation

Chromophore transformations in red fluorescent proteins.
Subach et al. (2012)
1 more