› 2vvm

X-ray diffraction
1.85Å resolution

The structure of MAO-N-D5, a variant of monoamine oxidase from Aspergillus niger.

Released:
DOI: 10.2210/pdb2vvm/pdb
PDB entry 2vvm coloured by chain, front view.
PDB entry 2vvm coloured by chain, side view.
PDB entry 2vvm coloured by chain, top view.
Source organism: Aspergillus niger
Primary publication:
The structure of monoamine oxidase from Aspergillus niger provides a molecular context for improvements in activity obtained by directed evolution.
Atkin KE, Reiss R, Koehler V, Bailey KR, Hart S, Turkenburg JP, Turner NJ, Brzozowski AM, Grogan G
J Mol Biol 384 1218-31 (2008)
PMID: 18951902

Function and Biology Details

Reaction catalysed: 
RCH(2)NHR' + H(2)O + O(2) = RCHO + R'NH(2) + H(2)O(2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155530 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Monoamine oxidase N Chains: A, B
Molecule details ›
Chains: A, B
Length: 495 amino acids
Theoretical weight: 55.58 KDa
Source organism: Aspergillus niger
Expression system: Escherichia coli
UniProt:
  • Canonical: P46882 (Residues: 1-495; Coverage: 100%)
Gene name: maoN
Sequence domains: Flavin containing amine oxidoreductase
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
2 bound ligands:
Ligand PRO 2 x PRO
Ligand EDO 6 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P43212
Unit cell:
a: 107.419Å b: 107.419Å c: 235.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.181 0.205
Expression system: Escherichia coli

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Citations

9 review citations

Directed evolution drives the next generation of biocatalysts.
Turner NJ. (2009)
8 more

7 mentions without citation

Cloning of a novel nicotine oxidase gene from Pseudomonas sp. strain HZN6 whose product nonenantioselectively degrades nicotine to pseudooxynicotine.
Qiu et al. (2013)
6 more