› 2vwk

X-ray diffraction
2.6Å resolution

Uracil Recognition in Archaeal DNA Polymerases Captured by X-ray Crystallography. V93Q polymerase variant

Released:
DOI: 10.2210/pdb2vwk/pdb
PDB entry 2vwk coloured by chain, front view.
PDB entry 2vwk coloured by chain, side view.
PDB entry 2vwk coloured by chain, top view.
Source organism: Thermococcus gorgonarius
Primary publication:
Uracil recognition in archaeal DNA polymerases captured by X-ray crystallography.
Firbank SJ, Wardle J, Heslop P, Lewis RJ, Connolly BA
J Mol Biol 381 529-39 (2008)
PMID: 18614176

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157523 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA polymerase Chain: A
Molecule details ›
Chain: A
Length: 773 amino acids
Theoretical weight: 89.97 KDa
Source organism: Thermococcus gorgonarius
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P56689 (Residues: 1-773; Coverage: 100%)
Gene names: pol, polA
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand NA 1 x NA
Ligand SO4 7 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 58.432Å b: 104.747Å c: 154.408Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.217 0.275
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

The eukaryotic replicative DNA polymerases take shape.
Johansson et al. (2010)
8 more

1 mention without citation

An archaeal family-B DNA polymerase variant able to replicate past DNA damage: occurrence of replicative and translesion synthesis polymerases within the B family.
Jozwiakowski et al. (2014)