› 2vy0

X-ray diffraction
2.16Å resolution

The X-ray structure of endo-beta-1,3-glucanase from Pyrococcus furiosus

Released:
DOI: 10.2210/pdb2vy0/pdb
PDB entry 2vy0 coloured by chain, front view.
PDB entry 2vy0 coloured by chain, side view.
PDB entry 2vy0 coloured by chain, top view.
Source organism: Pyrococcus furiosus
Primary publication:
Crystal structure of a family 16 endoglucanase from the hyperthermophile Pyrococcus furiosus--structural basis of substrate recognition.
Ilari A, Fiorillo A, Angelaccio S, Florio R, Chiaraluce R, van der Oost J, Consalvi V
FEBS J 276 1048-58 (2009)
PMID: 19154353

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130740 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GH16 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 264 amino acids
Theoretical weight: 30.14 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O73951 (Residues: 34-297; Coverage: 89%)
Gene name: lamA
Sequence domains: Glycosyl hydrolases family 16
Structure domains: Jelly Rolls

Ligands and Environments

5 bound ligands:
Ligand CL 3 x CL
Ligand NA 3 x NA
Ligand CA 2 x CA
Ligand MPD 2 x MPD
Ligand MRD 2 x MRD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P21
Unit cell:
a: 44.363Å b: 84.762Å c: 69.232Å
α: 90° β: 104.97° γ: 90°
R-values:
R R work R free
0.193 0.191 0.229
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

From Cancer Therapy to Winemaking: The Molecular Structure and Applications of β-Glucans and β-1, 3-Glucanases.
Caseiro et al. (2022)
13 other articles

10 mentions without citation

Comparative genomics allowed the identification of drug targets against human fungal pathogens.
Abadio et al. (2011)
9 more