› 2vye

X-ray diffraction
4.1Å resolution

Crystal Structure of the DnaC-ssDNA complex

Released:
DOI: 10.2210/pdb2vye/pdb
PDB entry 2vye coloured by chain, front view.
PDB entry 2vye coloured by chain, side view.
PDB entry 2vye coloured by chain, top view.
Source organisms:
Primary publication:
The crystal structure of a replicative hexameric helicase DnaC and its complex with single-stranded DNA.
Lo YH, Tsai KL, Sun YJ, Chen WT, Huang CY, Hsiao CD
Nucleic Acids Res 37 804-14 (2009)
PMID: 19074952

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115890 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Replicative DNA helicase Chains: A, B
Molecule details ›
Chains: A, B
Length: 454 amino acids
Theoretical weight: 50.83 KDa
Source organism: Geobacillus kaustophilus HTA426
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q5KU75 (Residues: 1-454; Coverage: 100%)
Gene name: GK3476
Sequence domains:
5'-D(*TP*TP*TP*TP*TP*TP*TP*TP*TP)-3' Chain: C
Molecule details ›
Chain: C
Length: 9 nucleotides
Theoretical weight: 2.69 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P63
Unit cell:
a: 180.83Å b: 180.83Å c: 104.112Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.302 0.302 0.374
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

10 review citations

Mechanisms for initiating cellular DNA replication.
Costa et al. (2013)
9 more

5 mentions without citation

Architecture and conservation of the bacterial DNA replication machinery, an underexploited drug target.
Robinson et al. (2012)
4 more