› 2vz0

X-ray diffraction
1.9Å resolution

Pteridine Reductase 1 (PTR1) from Trypanosoma Brucei in complex with NADP and DDD00066641

Released:
DOI: 10.2210/pdb2vz0/pdb
PDB entry 2vz0 coloured by chain, front view.
PDB entry 2vz0 coloured by chain, side view.
PDB entry 2vz0 coloured by chain, top view.
Source organism: Trypanosoma brucei brucei
Primary publication:
Development and validation of a cytochrome c-coupled assay for pteridine reductase 1 and dihydrofolate reductase.
Shanks EJ, Ong HB, Robinson DA, Thompson S, Sienkiewicz N, Fairlamb AH, Frearson JA
Anal Biochem 396 194-203 (2010)
PMID: 19748480

Function and Biology Details

Reaction catalysed: 
5,6,7,8-tetrahydrobiopterin + 2 NADP(+) = biopterin + 2 NADPH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131227 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pteridine reductase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 268 amino acids
Theoretical weight: 28.5 KDa
Source organism: Trypanosoma brucei brucei
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O76290 (Residues: 1-268; Coverage: 100%)
Gene name: PTR1
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAP 4 x NAP
1 bound ligand:
Ligand D64 4 x D64
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P21
Unit cell:
a: 74.479Å b: 90.822Å c: 82.616Å
α: 90° β: 115.6° γ: 90°
R-values:
R R work R free
0.156 0.154 0.203
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 citation in other articles

Trypanosoma brucei pteridine reductase 1 is essential for survival in vitro and for virulence in mice.
Sienkiewicz et al. (2010)
11 more

2 mentions without citation

The Potential of Secondary Metabolites from Plants as Drugs or Leads against Protozoan Neglected Diseases-Part III: In-Silico Molecular Docking Investigations.
Ogungbe et al. (2016)
1 more