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› 2w03

X-ray diffraction
2.95Å resolution

Co-complex Structure of Achromobactin Synthetase Protein D (AcsD) with adenosine, sulfate and citrate from Pectobacterium Chrysanthemi

Released:
DOI: 10.2210/pdb2w03/pdb
Model geometry
Fit model/data
PDB entry 2w03 coloured by chain, front view.
PDB entry 2w03 coloured by chain, side view.
PDB entry 2w03 coloured by chain, top view.
Source organism: Dickeya chrysanthemi
Primary publication:
AcsD catalyzes enantioselective citrate desymmetrization in siderophore biosynthesis.
Schmelz S, Kadi N, McMahon SA, Song L, Oves-Costales D, Oke M, Liu H, Johnson KA, Carter LG, Botting CH, White MF, Challis GL, Naismith JH
OpenAccess logo Nat Chem Biol 5 174-82 (2009)
PMID: 19182782

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188036 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AcsD Chains: A, B
Molecule details ›
Chains: A, B
Length: 620 amino acids
Theoretical weight: 70.6 KDa
Source organism: Dickeya chrysanthemi
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q93AT8 (Residues: 1-620; Coverage: 100%)
Gene name: acsD
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ADN 2 x ADN
Ligand SO4 2 x SO4
Ligand CIT 1 x CIT
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P1
Unit cell:
a: 57.65Å b: 69.14Å c: 94.19Å
α: 95.43° β: 101.45° γ: 95.06°
R-values:
R R work R free
0.205 0.202 0.267
Expression system: Escherichia coli BL21

Quick links

Citations

12 review citations

Chemistry and biology of siderophores.
Hider et al. (2010)
11 more

3 mentions without citation

Targeting adenylate-forming enzymes with designed sulfonyladenosine inhibitors.
Lux et al. (2019)
2 more