› 2w16

X-ray diffraction
2.71Å resolution

Structures of FpvA bound to heterologous pyoverdines

Released:
DOI: 10.2210/pdb2w16/pdb
PDB entry 2w16 coloured by chain, front view.
PDB entry 2w16 coloured by chain, side view.
PDB entry 2w16 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
FpvA bound to non-cognate pyoverdines: molecular basis of siderophore recognition by an iron transporter.
Greenwald J, Nader M, Celia H, Gruffaz C, Geoffroy V, Meyer JM, Schalk IJ, Pattus F
Mol Microbiol 72 1246-59 (2009)
PMID: 19504741

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155776 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ferripyoverdine receptor Chains: A, B
Molecule details ›
Chains: A, B
Length: 772 amino acids
Theoretical weight: 86.56 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Pseudomonas aeruginosa
UniProt:
  • Canonical: P48632 (Residues: 44-815; Coverage: 100%)
Gene names: PA2398, fpvA
Sequence domains:
Structure domains:
DSN-ARG-DSN-FHO-LYS-FHO-THR-THR Chain: C
Molecule details ›
Chain: C
Length: 8 amino acids
Theoretical weight: 997 Da
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Pseudomonas aeruginosa

Ligands and Environments

4 bound ligands:
Ligand N8E 2 x N8E
Ligand PO4 10 x PO4
Ligand PVE 1 x PVE
Ligand FE 1 x FE
2 modified residues:
Ligand DSN 2 x DSN
Ligand FHO 2 x FHO

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: C2
Unit cell:
a: 195.22Å b: 130.082Å c: 141.783Å
α: 90° β: 130.67° γ: 90°
R-values:
R R work R free
0.219 0.217 0.253
Expression system: Pseudomonas aeruginosa

Quick links

Citations

12 review citations

Microbial iron acquisition: marine and terrestrial siderophores.
Sandy et al. (2009)
11 more

4 mentions without citation

TonB-dependent transporters: regulation, structure, and function.
Noinaj et al. (2010)
3 more