› 2w1j

X-ray diffraction
1.24Å resolution

CRYSTAL STRUCTURE OF SORTASE C-1 (SRTC-1) from Streptococcus pneumoniae

Released:
DOI: 10.2210/pdb2w1j/pdb
PDB entry 2w1j coloured by chain, front view.
PDB entry 2w1j coloured by chain, side view.
PDB entry 2w1j coloured by chain, top view.
Source organism: Streptococcus pneumoniae TIGR4
Primary publication:
Sortase-mediated pilus fiber biogenesis in Streptococcus pneumoniae.
Manzano C, Contreras-Martel C, El Mortaji L, Izoré T, Fenel D, Vernet T, Schoehn G, Di Guilmi AM, Dessen A
Structure 16 1838-48 (2008)
PMID: 19081060

Function and Biology Details

Reaction catalysed: 
Peptidyl-tRNA(1) + aminoacyl-tRNA(2) = tRNA(1) + peptidyl(aminoacyl-tRNA(2))
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101410 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative sortase Chains: A, B
Molecule details ›
Chains: A, B
Length: 212 amino acids
Theoretical weight: 23.76 KDa
Source organism: Streptococcus pneumoniae TIGR4
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0H2UNQ9 (Residues: 17-228; Coverage: 76%)
Gene name: SP_0466
Sequence domains: Sortase domain
Structure domains: Sortase

Ligands and Environments

1 bound ligand:
Ligand GOL 6 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 68.091Å b: 70.189Å c: 86.542Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.171 0.185
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Architects at the bacterial surface - sortases and the assembly of pili with isopeptide bonds.
Hendrickx et al. (2011)
13 more

10 mentions without citation

Sortase transpeptidases: insights into mechanism, substrate specificity, and inhibition.
Clancy et al. (2010)
9 more