› 2w1s

X-ray diffraction
1.45Å resolution

Unique ligand binding specificity of a family 32 Carbohydrate-Binding Module from the Mu toxin produced by Clostridium perfringens

Released:
DOI: 10.2210/pdb2w1s/pdb
PDB entry 2w1s coloured by chain, front view.
PDB entry 2w1s coloured by chain, side view.
PDB entry 2w1s coloured by chain, top view.
Source organism: Clostridium perfringens
Primary publication:
N-acetylglucosamine recognition by a family 32 carbohydrate-binding module from Clostridium perfringens NagH.
Ficko-Blean E, Boraston AB
J Mol Biol 390 208-20 (2009)
PMID: 19422833

Function and Biology Details

Reaction catalysed: 
Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150857 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hyaluronoglucosaminidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 192 amino acids
Theoretical weight: 21.54 KDa
Source organism: Clostridium perfringens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P26831 (Residues: 801-975; Coverage: 11%)
Gene names: CPE0191, nagH
Sequence domains: F5/8 type C domain
Structure domains: Galactose-binding domain-like

Ligands and Environments

3 bound ligands:
Ligand CA 2 x CA
Ligand NA 2 x NA
Ligand BTB 1 x BTB
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 56.325Å b: 61.847Å c: 82.993Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.174 0.226
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Carbohydrate-binding domains: multiplicity of biological roles.
Guillén et al. (2010)
3 more

5 mentions without citation

N-acetylglucosamine recognition by a family 32 carbohydrate-binding module from Clostridium perfringens NagH.
Ficko-Blean et al. (2009)
4 more