› 2w2h

X-ray diffraction
3.25Å resolution

Structural basis of transcription activation by the Cyclin T1-Tat-TAR RNA complex from EIAV

Released:
DOI: 10.2210/pdb2w2h/pdb
PDB entry 2w2h coloured by chain, front view.
PDB entry 2w2h coloured by chain, side view.
PDB entry 2w2h coloured by chain, top view.
Source organisms:
Primary publication:
Structural insights into the cyclin T1-Tat-TAR RNA transcription activation complex from EIAV.
Anand K, Schulte A, Vogel-Bachmayr K, Scheffzek K, Geyer M
Nat Struct Mol Biol 15 1287-92 (2008)
PMID: 19029897

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149088 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (3 distinct):
Cyclin-T1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 264 amino acids
Theoretical weight: 30.84 KDa
Source organism: Equus caballus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9XT26 (Residues: 4-267; Coverage: 36%)
Gene name: CCNT1
Sequence domains:
Structure domains: Cyclin-like
Protein Tat Chains: C, D
Molecule details ›
Chains: C, D
Length: 29 amino acids
Theoretical weight: 3.49 KDa
Source organism: Equine infectious anemia virus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P20920 (Residues: 47-75; Coverage: 39%)
Gene name: tat
Sequence domains: Transactivating regulatory protein (Tat)
5'-R(*GP*CP*UP*CP*AP*GP*AP*UP*CP*UP *GP*CP*GP*GP*UP*CP*UP*GP*AP*GP*C)-3' Chains: R, S
Molecule details ›
Chains: R, S
Length: 22 nucleotides
Theoretical weight: 7.03 KDa

Ligands and Environments

1 bound ligand:
Ligand MN 2 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: I4
Unit cell:
a: 149.46Å b: 149.46Å c: 129.75Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.245 0.243 0.278
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

The structural biology of HIV-1: mechanistic and therapeutic insights.
Engelman et al. (2012)
7 more

8 mentions without citation

The control of HIV transcription: keeping RNA polymerase II on track.
Ott et al. (2011)
7 more