› 2w3v

X-ray diffraction
1.89Å resolution

MYCOBACTERIUM AVIUM DIHYDROFOLATE REDUCTASE COMPLEXED WITH NADPH AND TRIMETHOPRIM

Released:
DOI: 10.2210/pdb2w3v/pdb
PDB entry 2w3v coloured by chain, front view.
PDB entry 2w3v coloured by chain, side view.
PDB entry 2w3v coloured by chain, top view.
Source organism: Mycobacterium avium
Entry authors: Leung AKW, Reynolds RC, Borhani DW

Function and Biology Details

Reaction catalysed: 
5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128247 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydrofolate reductase Chain: A
Molecule details ›
Chain: A
Length: 167 amino acids
Theoretical weight: 18.45 KDa
Source organism: Mycobacterium avium
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O30463 (Residues: 1-167; Coverage: 92%)
Gene name: folA
Sequence domains: Dihydrofolate reductase
Structure domains: Dihydrofolate Reductase, subunit A

Ligands and Environments


Cofactor: Ligand NDP 1 x NDP
1 bound ligand:
Ligand TOP 1 x TOP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P41212
Unit cell:
a: 70.816Å b: 70.816Å c: 73.613Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.18 0.224
Expression system: Escherichia coli BL21(DE3)

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