› 2w42

X-ray diffraction
1.9Å resolution

THE STRUCTURE OF A PIWI PROTEIN FROM ARCHAEOGLOBUS FULGIDUS COMPLEXED WITH A 16NT DNA DUPLEX.

Released:
DOI: 10.2210/pdb2w42/pdb
PDB entry 2w42 coloured by chain, front view.
PDB entry 2w42 coloured by chain, side view.
PDB entry 2w42 coloured by chain, top view.
Source organisms:
Primary publication:
Enhancement of the seed-target recognition step in RNA silencing by a PIWI/MID domain protein.
Parker JS, Parizotto EA, Wang M, Roe SM, Barford D
Mol Cell 33 204-14 (2009)
PMID: 19187762

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115895 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Piwi protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 427 amino acids
Theoretical weight: 49.3 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O28951 (Residues: 1-427; Coverage: 100%)
Gene name: AF_1318
Sequence domains: Piwi domain
Structure domains:
5'-D(*TP*TP*CP*GP*AP*CP*GP*CP)-3' Chains: P, R
Molecule details ›
Chains: P, R
Length: 8 nucleotides
Theoretical weight: 2.4 KDa
Source organism: Homo sapiens
Expression system: Not provided
5'-D(*GP*TP*CP*GP*AP*AP*TP*TP)-3' Chains: Q, S
Molecule details ›
Chains: Q, S
Length: 8 nucleotides
Theoretical weight: 2.44 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MN 2 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P1
Unit cell:
a: 51.838Å b: 61.384Å c: 103.54Å
α: 75.8° β: 75.86° γ: 79.47°
R-values:
R R work R free
0.207 0.205 0.243
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

22 review citations

Regulation of mRNA translation and stability by microRNAs.
Fabian et al. (2010)
21 more

6 mentions without citation

DNA interference and beyond: structure and functions of prokaryotic Argonaute proteins.
Lisitskaya et al. (2018)
5 more