› 2w45

X-ray diffraction
3Å resolution

Epstein-Barr virus alkaline nuclease

Released:
DOI: 10.2210/pdb2w45/pdb
PDB entry 2w45 coloured by chain, front view.
PDB entry 2w45 coloured by chain, side view.
PDB entry 2w45 coloured by chain, top view.
Source organism: Human gammaherpesvirus 4
Primary publication:
A bridge crosses the active-site canyon of the Epstein-Barr virus nuclease with DNase and RNase activities.
Buisson M, Géoui T, Flot D, Tarbouriech N, Ressing ME, Wiertz EJ, Burmeister WP
J Mol Biol 391 717-28 (2009)
PMID: 19538972

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136663 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Shutoff alkaline exonuclease Chains: A, B
Molecule details ›
Chains: A, B
Length: 470 amino acids
Theoretical weight: 52.72 KDa
Source organism: Human gammaherpesvirus 4
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P03217 (Residues: 1-470; Coverage: 100%)
Gene name: BGLF5
Sequence domains: Viral alkaline exonuclease
Structure domains: Herpesvirus alkaline exonuclease, N-terminal domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P21
Unit cell:
a: 87.51Å b: 63.787Å c: 114.131Å
α: 90° β: 93.59° γ: 90°
R-values:
R R work R free
0.197 0.192 0.282
Expression system: Trichoplusia ni

Quick links

Citations

12 review citations

Herpesviruses and immunity: the art of evasion.
Griffin et al. (2010)
11 more

2 mentions without citation

Immune responses to Epstein-Barr virus: molecular interactions in the virus evasion of CD8+ T cell immunity.
Rowe et al. (2010)
1 more