› 2w4g

Electron Microscopy
35Å resolution

ISOMETRICALLY CONTRACTING INSECT ASYNCHRONOUS FLIGHT MUSCLE QUICK FROZEN AFTER A QUICK STRETCH STEP

Released:
DOI: 10.2210/pdb2w4g/pdb
PDB entry 2w4g coloured by chain, ensemble of 20 models, front view.
PDB entry 2w4g coloured by chain, ensemble of 20 models, side view.
PDB entry 2w4g coloured by chain, ensemble of 20 models, top view.
Source organism: Gallus gallus
Primary publication:
Electron tomography of cryofixed, isometrically contracting insect flight muscle reveals novel actin-myosin interactions.
Wu S, Liu J, Reedy MC, Tregear RT, Winkler H, Franzini-Armstrong C, Sasaki H, Lucaveche C, Goldman YE, Reedy MK, Taylor KA
PLoS One 5 (2010)
PMID: 20844746
Related structures: EMD-1584

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135952 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Myosin regulatory light chain 11 Chain: B
Molecule details ›
Chain: B
Length: 150 amino acids
Theoretical weight: 16.96 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: P02609 (Residues: 16-165; Coverage: 89%)
Gene names: MYL11, MYLPF, RLC
Myosin light chain 3, skeletal muscle isoform Chain: C
Molecule details ›
Chain: C
Length: 145 amino acids
Theoretical weight: 16.18 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: P02605 (Residues: 6-150; Coverage: 97%)
Myosin heavy chain, skeletal muscle, adult Chain: M
Molecule details ›
Chain: M
Length: 840 amino acids
Theoretical weight: 95.9 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: P13538 (Residues: 5-844; Coverage: 43%)
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 35Å
Relevant EMDB volumes: EMD-1584

Quick links

Citations

11 review citations

Regulating the contraction of insect flight muscle.
Bullard et al. (2011)
10 more

2 mentions without citation

Structural changes in isometrically contracting insect flight muscle trapped following a mechanical perturbation.
Wu et al. (2012)
1 more