› 2w4s

X-ray diffraction
2.45Å resolution

novel RNA-binding domain in Cryptosporidium parvum at 2.5 angstrom resolution

Released:
DOI: 10.2210/pdb2w4s/pdb
PDB entry 2w4s coloured by chain, front view.
PDB entry 2w4s coloured by chain, side view.
PDB entry 2w4s coloured by chain, top view.
Source organism: Cryptosporidium parvum
Primary publication:
Structure and RNA recognition by the snRNA and snoRNA transport factor PHAX.
Mourão A, Varrot A, Mackereth CD, Cusack S, Sattler M
RNA 16 1205-16 (2010)
PMID: 20430857

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163281 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphorylated adapter RNA export protein RNA-binding domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 113 amino acids
Theoretical weight: 13.01 KDa
Source organism: Cryptosporidium parvum
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A0A7G2HK26 (Residues: 387-496; Coverage: 22%)
Gene names: 1MB.655, CPATCC_003027
Sequence domains: Phosphorylated adapter RNA export protein, RNA-binding domain
Structure domains: PHAX RNA-binding domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21
Unit cell:
a: 42.18Å b: 52.862Å c: 99.315Å
α: 90° β: 94.05° γ: 90°
R-values:
R R work R free
0.196 0.193 0.254
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

Structure determination and dynamics of protein-RNA complexes by NMR spectroscopy.
Dominguez et al. (2011)
1 more

1 mention without citation

Structure and RNA recognition by the snRNA and snoRNA transport factor PHAX.
Mourão et al. (2010)