› 2w52

X-ray diffraction
1.56Å resolution

2 beta-glucans (6-O-glucosyl-laminaritriose) in both donor and acceptor sites of GH16 Laminarinase 16A from Phanerochaete chrysosporium.

Released:
DOI: 10.2210/pdb2w52/pdb
PDB entry 2w52 coloured by chain, front view.
PDB entry 2w52 coloured by chain, side view.
PDB entry 2w52 coloured by chain, top view.
Source organism: Phanerodontia chrysosporium
Primary publication:
X-ray crystal structures of Phanerochaete chrysosporium Laminarinase 16A in complex with products from lichenin and laminarin hydrolysis.
Vasur J, Kawai R, Andersson E, Igarashi K, Sandgren M, Samejima M, Ståhlberg J
FEBS J 276 3858-69 (2009)
PMID: 19769746

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->3)- or (1->4)-linkages in beta-D-glucans when the glucose residue whose reducing group is involved in the linkage to be hydrolyzed is itself substituted at C-3.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183292 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
GH16 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 298 amino acids
Theoretical weight: 31.92 KDa
Source organism: Phanerodontia chrysosporium
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q874E3 (Residues: 21-318; Coverage: 100%)
Gene name: lam16A
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-2
Spacegroup: P212121
Unit cell:
a: 38.056Å b: 46.69Å c: 152.871Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.152 0.151 0.171
Expression system: Komagataella pastoris

Quick links

Citations

1 review citation

3D Structural Insights into β-Glucans and Their Binding Proteins.
Manabe et al. (2021)
17 other articles

1 mention without citation

Bioinformatics and molecular modeling in glycobiology.
Frank et al. (2010)