› 2w5e

X-ray diffraction
2Å resolution

Structural and biochemical analysis of human pathogenic astrovirus serine protease at 2.0 Angstrom resolution

Released:
DOI: 10.2210/pdb2w5e/pdb
PDB entry 2w5e coloured by chain, front view.
PDB entry 2w5e coloured by chain, side view.
PDB entry 2w5e coloured by chain, top view.
Source organism: Human astrovirus 1
Primary publication:
Structural and biochemical analysis of human pathogenic astrovirus serine protease at 2.0 A resolution.
Speroni S, Rohayem J, Nenci S, Bonivento D, Robel I, Barthel J, Luzhkov VB, Coutard B, Canard B, Mattevi A
J Mol Biol 387 1137-52 (2009)
PMID: 19249313

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174914 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative serine protease Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 163 amino acids
Theoretical weight: 17.69 KDa
Source organism: Human astrovirus 1
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q3ZN04 (Residues: 432-587; Coverage: 17%)
Sequence domains: Trypsin-like peptidase domain
Structure domains: Trypsin-like serine proteases

Ligands and Environments

2 bound ligands:
Ligand CD 18 x CD
Ligand CL 28 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P3121
Unit cell:
a: 135.047Å b: 135.047Å c: 189.955Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.234 0.234 0.258
Expression system: Escherichia coli BL21

Quick links

Citations

3 review citations

Astrovirus infections in humans and animals - molecular biology, genetic diversity, and interspecies transmissions.
De Benedictis et al. (2011)
2 more

4 mentions without citation

Characterization of Bafinivirus main protease autoprocessing activities.
Ulferts et al. (2011)
3 more