› 2w62

X-ray diffraction
1.85Å resolution

Saccharomyces cerevisiae Gas2p in complex with laminaripentaose

Released:
DOI: 10.2210/pdb2w62/pdb
PDB entry 2w62 coloured by chain, front view.
PDB entry 2w62 coloured by chain, side view.
PDB entry 2w62 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Molecular mechanisms of yeast cell wall glucan remodeling.
Hurtado-Guerrero R, Schüttelkopf AW, Mouyna I, Ibrahim AF, Shepherd S, Fontaine T, Latgé JP, van Aalten DM
J Biol Chem 284 8461-9 (2009)
PMID: 19097997

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170293 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
1,3-beta-glucanosyltransferase GAS2 Chain: A
Molecule details ›
Chain: A
Length: 555 amino acids
Theoretical weight: 62.43 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q06135 (Residues: 1-555; Coverage: 100%)
Gene names: GAS2, L8300.5, YLR343W
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
1 bound ligand:
Ligand BU1 1 x BU1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 50.039Å b: 70.841Å c: 149.149Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.183 0.21
Expression system: Komagataella pastoris

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Citations

7 review citations

Aspergillus fumigatus: cell wall polysaccharides, their biosynthesis and organization.
Gastebois et al. (2009)
6 more

1 mention without citation

Molecular mechanisms of yeast cell wall glucan remodeling.
Hurtado-Guerrero et al. (2009)