› 2w79

X-ray diffraction
1.85Å resolution

Establishing wild-type levels of catalytic activity on natural and artificial (ba)8-barrel protein scaffolds

Released:
DOI: 10.2210/pdb2w79/pdb
PDB entry 2w79 coloured by chain, front view.
PDB entry 2w79 coloured by chain, side view.
PDB entry 2w79 coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
Establishing wild-type levels of catalytic activity on natural and artificial (beta alpha)8-barrel protein scaffolds.
Claren J, Malisi C, Höcker B, Sterner R
Proc Natl Acad Sci U S A 106 3704-9 (2009)
PMID: 19237570

Function and Biology Details

Reaction catalysed: 
1-(5-phospho-beta-D-ribosyl)-5-((5-phospho-beta-D-ribosylamino)methylideneamino)imidazole-4-carboxamide = 5-((5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino)-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194695 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 241 amino acids
Theoretical weight: 27 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9X0C7 (Residues: 1-241; Coverage: 100%)
Gene names: TM_1037, hisA
Sequence domains: Histidine biosynthesis protein
Structure domains: Aldolase class I

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 49Å b: 64.1Å c: 79.2Å
α: 90° β: 94° γ: 90°
R-values:
R R work R free
0.206 0.202 0.267
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Exploring protein fitness landscapes by directed evolution.
Romero et al. (2009)
11 more

2 mentions without citation

Establishing wild-type levels of catalytic activity on natural and artificial (beta alpha)8-barrel protein scaffolds.
Claren et al. (2009)
1 more