› 2w7n

X-ray diffraction
1.85Å resolution

Crystal Structure of KorA Bound to Operator DNA: Insight into Repressor Cooperation in RP4 Gene Regulation

Released:
DOI: 10.2210/pdb2w7n/pdb
PDB entry 2w7n coloured by chain, front view.
PDB entry 2w7n coloured by chain, side view.
PDB entry 2w7n coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of KorA bound to operator DNA: insight into repressor cooperation in RP4 gene regulation.
König B, Müller JJ, Lanka E, Heinemann U
Nucleic Acids Res 37 1915-24 (2009)
PMID: 19190096

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115896 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
TrfB transcriptional repressor protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 101 amino acids
Theoretical weight: 11.32 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03052 (Residues: 1-101; Coverage: 100%)
Gene names: korA, trfB
Sequence domains: TrfB plasmid transcriptional repressor
Structure domains: Arc Repressor Mutant, subunit A
5'-D(*AP*AP*TP*GP*TP*TP*TP*AP*GP*CP *TP*AP*AP*AP*CP*AP*AP*G)-3' Chains: E, G
Molecule details ›
Chains: E, G
Length: 18 nucleotides
Theoretical weight: 5.55 KDa
Source organism: Escherichia coli
Expression system: Not provided
5'-D(*CP*BRUP*BRUP*GP*TP*TP*TP*AP*GP*CP*TP*AP *AP*AP*CP*AP*BRUP*T)-3' Chains: F, H
Molecule details ›
Chains: F, H
Length: 18 nucleotides
Theoretical weight: 5.68 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand BRU 6 x BRU

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P21
Unit cell:
a: 44.83Å b: 115.45Å c: 49.88Å
α: 90° β: 114° γ: 90°
R-values:
R R work R free
0.172 0.17 0.201
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

2 review citations

Origins of specificity in protein-DNA recognition.
Rohs et al. (2010)
1 more

4 mentions without citation

Crystal structure of KorA bound to operator DNA: insight into repressor cooperation in RP4 gene regulation.
König et al. (2009)
3 more