› 2w7y

X-ray diffraction
2.35Å resolution

Structure of a Streptococcus pneumoniae solute-binding protein in complex with the blood group A-trisaccharide.

Released:
DOI: 10.2210/pdb2w7y/pdb
PDB entry 2w7y coloured by chain, front view.
PDB entry 2w7y coloured by chain, side view.
PDB entry 2w7y coloured by chain, top view.
Source organism: Streptococcus pneumoniae SP3-BS71
Primary publication:
Blood group antigen recognition by a solute-binding protein from a serotype 3 strain of Streptococcus pneumoniae.
Higgins MA, Abbott DW, Boulanger MJ, Boraston AB
J Mol Biol 388 299-309 (2009)
PMID: 19285508

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163287 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
PROBABLE SUGAR ABC TRANSPORTER, SUGAR-BINDING PROTEIN Chains: A, B
Molecule details ›
Chains: A, B
Length: 430 amino acids
Theoretical weight: 47.17 KDa
Source organism: Streptococcus pneumoniae SP3-BS71
Expression system: Escherichia coli BL21(DE3)
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

Carbohydrate polymer : NEW Components: GAL, FUC, A2G
Carbohydrate polymer
1 bound ligand:
Ligand IOD 2 x IOD
1 modified residue:
Ligand MSE 22 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P21
Unit cell:
a: 36.99Å b: 104.9Å c: 97.7Å
α: 90° β: 89.95° γ: 90°
R-values:
R R work R free
0.216 0.213 0.263
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Pneumococcal carbohydrate transport: food for thought.
Buckwalter et al. (2012)
2 more

3 mentions without citation

Biochemical and structural insights into xylan utilization by the thermophilic bacterium Caldanaerobius polysaccharolyticus.
Han et al. (2012)
2 more