› 2w8h

X-ray diffraction
2.76Å resolution

Crystal structure of spin labeled Wza24-345.

Released:
DOI: 10.2210/pdb2w8h/pdb
PDB entry 2w8h coloured by chain, front view.
PDB entry 2w8h coloured by chain, side view.
PDB entry 2w8h coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
PELDOR spectroscopy distance fingerprinting of the octameric outer-membrane protein Wza from Escherichia coli.
Hagelueken G, Ingledew WJ, Huang H, Petrovic-Stojanovska B, Whitfield C, ElMkami H, Schiemann O, Naismith JH
Angew Chem Int Ed Engl 48 2904-6 (2009)
PMID: 19294709

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194907 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative outer membrane lipoprotein Wza Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 329 amino acids
Theoretical weight: 35.87 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9X4B7 (Residues: 22-345; Coverage: 86%)
Gene name: wza
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MTN 8 x MTN
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21212
Unit cell:
a: 141.153Å b: 141.152Å c: 167.529Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.211 0.229
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Conformational dynamics and distribution of nitroxide spin labels.
Jeschke G. (2013)
4 more

2 mentions without citation

MtsslWizard: In Silico Spin-Labeling and Generation of Distance Distributions in PyMOL.
Hagelueken et al. (2012)
1 more