› 2wa8

X-ray diffraction
2.15Å resolution

Structural basis of N-end rule substrate recognition in Escherichia coli by the ClpAP adaptor protein ClpS - The Phe peptide structure

Released:
DOI: 10.2210/pdb2wa8/pdb
PDB entry 2wa8 coloured by chain, front view.
PDB entry 2wa8 coloured by chain, side view.
PDB entry 2wa8 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of N-end rule substrate recognition in Escherichia coli by the ClpAP adaptor protein ClpS.
Schuenemann VJ, Kralik SM, Albrecht R, Spall SK, Truscott KN, Dougan DA, Zeth K
EMBO Rep 10 508-14 (2009)
PMID: 19373253

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141729 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
ATP-dependent Clp protease adapter protein ClpS Chains: A, C
Molecule details ›
Chains: A, C
Length: 107 amino acids
Theoretical weight: 12.34 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0A8Q6 (Residues: 1-106; Coverage: 100%)
Gene names: JW0865, b0881, clpS, yljA
Sequence domains: ATP-dependent Clp protease adaptor protein ClpS
Structure domains: Ribosomal Protein L30; Chain: A,
N-END RULE PEPTIDE Chains: B, D
Molecule details ›
Chains: B, D
Length: 10 amino acids
Theoretical weight: 1.22 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 32.223Å b: 58.405Å c: 56.413Å
α: 90° β: 101.89° γ: 90°
R-values:
R R work R free
0.228 0.225 0.268
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

20 review citations

The N-end rule pathway and regulation by proteolysis.
Varshavsky A. (2011)
19 more

1 mention without citation

The ClpS adaptor mediates staged delivery of N-end rule substrates to the AAA+ ClpAP protease.
Román-Hernández et al. (2011)