› 2wae

X-ray diffraction
2.26Å resolution

PENICILLIN-BINDING PROTEIN 2B (PBP-2B) FROM STREPTOCOCCUS PNEUMONIAE (STRAIN 5204)

Released:
DOI: 10.2210/pdb2wae/pdb
PDB entry 2wae coloured by chain, front view.
PDB entry 2wae coloured by chain, side view.
PDB entry 2wae coloured by chain, top view.
Source organism: Streptococcus pneumoniae
Primary publication:
PBP active site flexibility as the key mechanism for beta-lactam resistance in pneumococci.
Contreras-Martel C, Dahout-Gonzalez C, Martins Ados S, Kotnik M, Dessen A
J Mol Biol 387 899-909 (2009)
PMID: 19233207

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141214 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Penicillin-binding protein 2B Chain: A
Molecule details ›
Chain: A
Length: 680 amino acids
Theoretical weight: 73.83 KDa
Source organism: Streptococcus pneumoniae
UniProt:
  • Canonical: P0A3M6 (Residues: 1-680; Coverage: 100%)
Gene names: pbp2b, penA, spr1517
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: R32
Unit cell:
a: 127.951Å b: 127.951Å c: 280.847Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.247 0.244 0.301

Quick links

Citations

8 review citations

Biological and Epidemiological Features of Antibiotic-Resistant Streptococcus pneumoniae in Pre- and Post-Conjugate Vaccine Eras: a United States Perspective.
Kim et al. (2016)
7 more

2 mentions without citation

β-Lactam Resistance Mechanisms: Gram-Positive Bacteria and Mycobacterium tuberculosis.
Fisher et al. (2016)
1 more