› 2wan

X-ray diffraction
1.65Å resolution

Pullulanase from Bacillus acidopullulyticus

Released:
DOI: 10.2210/pdb2wan/pdb
PDB entry 2wan coloured by chain, front view.
PDB entry 2wan coloured by chain, side view.
PDB entry 2wan coloured by chain, top view.
Source organism: Bacillus acidopullulyticus
Primary publication:
Structure of a pullulanase from Bacillus acidopullulyticus.
Turkenburg JP, Brzozowski AM, Svendsen A, Borchert TV, Davies GJ, Wilson KS
Proteins 76 516-9 (2009)
PMID: 19382205

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163290 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PULLULANASE Chain: A
Molecule details ›
Chain: A
Length: 921 amino acids
Theoretical weight: 101.63 KDa
Source organism: Bacillus acidopullulyticus
Expression system: Bacillus subtilis
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand GOL 8 x GOL
Ligand SO4 4 x SO4
Ligand ACT 4 x ACT
1 modified residue:
Ligand CSX 1 x CSX

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: C2
Unit cell:
a: 163.431Å b: 61.983Å c: 109.668Å
α: 90° β: 109.1° γ: 90°
R-values:
R R work R free
0.177 0.175 0.21
Expression system: Bacillus subtilis

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Citations

6 review citations

Collaborative actions in anti-trypanosomatid chemotherapy with partners from disease endemic areas.
Dujardin et al. (2010)
5 more

13 mentions without citation

The Sus operon: a model system for starch uptake by the human gut Bacteroidetes.
Foley et al. (2016)
12 more