› 2wcd

X-ray diffraction
3.29Å resolution

Crystal structure of the assembled cytolysin A pore

Released:
DOI: 10.2210/pdb2wcd/pdb
PDB entry 2wcd coloured by chain, front view.
PDB entry 2wcd coloured by chain, side view.
PDB entry 2wcd coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
The structure of a cytolytic alpha-helical toxin pore reveals its assembly mechanism.
Mueller M, Grauschopf U, Maier T, Glockshuber R, Ban N
Nature 459 726-30 (2009)
PMID: 19421192

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assemblies composition:
homo 24-mer
homo dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160150 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hemolysin E, chromosomal Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X
Length: 309 amino acids
Theoretical weight: 34.62 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P77335 (Residues: 2-303; Coverage: 100%)
Gene names: JW5181, b1182, clyA, hlyE, hpr, sheA, ycgD
Sequence domains: Haemolysin E (HlyE)
Structure domains: Hemolysin E; Chain: A;

Ligands and Environments

1 bound ligand:
Ligand EMC 48 x EMC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P1
Unit cell:
a: 111.43Å b: 114.41Å c: 270.55Å
α: 94.44° β: 85.92° γ: 102.55°
R-values:
R R work R free
0.228 0.228 0.244
Expression system: Escherichia coli

Quick links

Citations

37 review citations

Botulinum neurotoxin: a marvel of protein design.
Montal M. (2010)
36 more

38 mentions without citation

Mechanism of function of viral channel proteins and implications for drug development.
Fischer et al. (2012)
37 more