› 2wci

X-ray diffraction
1.9Å resolution

Structure of E. coli monothiol glutaredoxin GRX4 homodimer

Released:
DOI: 10.2210/pdb2wci/pdb
PDB entry 2wci coloured by chain, front view.
PDB entry 2wci coloured by chain, side view.
PDB entry 2wci coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural basis for delivery of the intact [Fe2S2] cluster by monothiol glutaredoxin.
Iwema T, Picciocchi A, Traore DA, Ferrer JL, Chauvat F, Jacquamet L
Biochemistry 48 6041-3 (2009)
PMID: 19505088

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142246 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutaredoxin 4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 135 amino acids
Theoretical weight: 15.06 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AC69 (Residues: 1-115; Coverage: 100%)
Gene names: JW1646, b1654, grxD, ydhD
Sequence domains: Glutaredoxin
Structure domains: Glutaredoxin

Ligands and Environments

3 bound ligands:
Ligand FES 1 x FES
Ligand GSH 2 x GSH
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P43212
Unit cell:
a: 94.3Å b: 94.3Å c: 62.66Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.183 0.234
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

20 review citations

Glutathione catalysis and the reaction mechanisms of glutathione-dependent enzymes.
Deponte M. (2013)
19 more

14 mentions without citation

Monothiol CGFS glutaredoxins and BolA-like proteins: [2Fe-2S] binding partners in iron homeostasis.
Li et al. (2012)
13 more