› 2wcw

X-ray diffraction
1.58Å resolution

1.6A resolution structure of Archaeoglobus fulgidus Hjc, a Holliday junction resolvase from an archaeal hyperthermophile

Released:
DOI: 10.2210/pdb2wcw/pdb
PDB entry 2wcw coloured by chain, front view.
PDB entry 2wcw coloured by chain, side view.
PDB entry 2wcw coloured by chain, top view.
Source organism: Archaeoglobus fulgidus
Entry authors: Carolis C, Koehler C, Sauter C, Basquin J, Suck D, Toeroe I

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128010 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Crossover junction endodeoxyribonuclease Hjc Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 139 amino acids
Theoretical weight: 15.54 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O28314 (Residues: 2-136; Coverage: 99%)
Gene names: AF_1965, hjc
Sequence domains: Archaeal holliday junction resolvase (hjc)
Structure domains: Trna Endonuclease; Chain: A, domain 1

Ligands and Environments

2 bound ligands:
Ligand ACT 6 x ACT
Ligand NH4 5 x NH4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 57.51Å b: 75.48Å c: 60.92Å
α: 90° β: 115.34° γ: 90°
R-values:
R R work R free
0.156 0.154 0.19
Expression system: Escherichia coli BL21(DE3)

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