› 2wd0

X-ray diffraction
2.74Å resolution

CRYSTAL STRUCTURE OF NONSYNDROMIC DEAFNESS (DFNB12) ASSOCIATED MUTANT D124G OF MOUSE CADHERIN-23 EC1-2

Released:
DOI: 10.2210/pdb2wd0/pdb
PDB entry 2wd0 coloured by chain, front view.
PDB entry 2wd0 coloured by chain, side view.
PDB entry 2wd0 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural determinants of cadherin-23 function in hearing and deafness.
Sotomayor M, Weihofen WA, Gaudet R, Corey DP
Neuron 66 85-100 (2010)
PMID: 20399731

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189374 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cadherin-23 Chains: A, C
Molecule details ›
Chains: A, C
Length: 214 amino acids
Theoretical weight: 23.8 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q99PF4 (Residues: 24-228; Coverage: 6%)
Gene name: Cdh23
Sequence domains: Cadherin domain
Structure domains: Cadherins

Ligands and Environments

3 bound ligands:
Ligand CA 6 x CA
Ligand NA 2 x NA
Ligand CL 3 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P42212
Unit cell:
a: 179.693Å b: 179.693Å c: 63.982Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.219 0.249
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

24 review citations

Thinking outside the cell: how cadherins drive adhesion.
Brasch et al. (2012)
23 more

3 mentions without citation

Structural determinants of cadherin-23 function in hearing and deafness.
Sotomayor et al. (2010)
2 more