› 2wdd

X-ray diffraction
1.5Å resolution

Termus thermophilus Sulfate thiohydrolase SoxB in complex with Sulfate

Released:
DOI: 10.2210/pdb2wdd/pdb
PDB entry 2wdd coloured by chain, front view.
PDB entry 2wdd coloured by chain, side view.
PDB entry 2wdd coloured by chain, top view.
Source organism: Thermus thermophilus HB27
Primary publication:
Mechanism for the hydrolysis of a sulfur-sulfur bond based on the crystal structure of the thiosulfohydrolase SoxB.
Sauvé V, Roversi P, Leath KJ, Garman EF, Antrobus R, Lea SM, Berks BC
J Biol Chem 284 21707-18 (2009)
PMID: 19535341

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180844 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5'-Nucleotidase C-terminal domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 562 amino acids
Theoretical weight: 62.75 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli
UniProt:
  • Canonical: Q72IT0 (Residues: 18-573; Coverage: 97%)
Gene names: TT_C1052, soxB
Sequence domains: 5'-nucleotidase, C-terminal domain
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand ACT 1 x ACT
Ligand TBU 3 x TBU
Ligand SO4 1 x SO4
Ligand MN 2 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 70.834Å b: 86.618Å c: 96.095Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.17 0.196
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Sulfur Oxidation in the Acidophilic Autotrophic Acidithiobacillus spp.
Wang et al. (2018)
4 more

1 mention without citation

Mechanism for the hydrolysis of a sulfur-sulfur bond based on the crystal structure of the thiosulfohydrolase SoxB.
Sauvé et al. (2009)