› 2wfk

X-ray diffraction
2.3Å resolution

Calcium bound LipL32

Released:
DOI: 10.2210/pdb2wfk/pdb
PDB entry 2wfk coloured by chain, front view.
PDB entry 2wfk coloured by chain, side view.
PDB entry 2wfk coloured by chain, top view.
Source organism: Leptospira santarosai serovar Shermani
Primary publication:
Calcium binds to LipL32, a lipoprotein from pathogenic Leptospira, and modulates fibronectin binding.
Tung JY, Yang CW, Chou SW, Lin CC, Sun YJ
J Biol Chem 285 3245-52 (2010)
PMID: 19948735

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180321 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Surface lipoprotein of Spirochaetales order domain-containing protein Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 259 amino acids
Theoretical weight: 28.79 KDa
Source organism: Leptospira santarosai serovar Shermani
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6S9S1 (Residues: 20-266; Coverage: 100%)
Gene name: lipL32
Sequence domains: Surface lipoprotein of Spirochaetales order

Ligands and Environments

1 bound ligand:
Ligand CA 5 x CA
1 modified residue:
Ligand MSE 25 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL12B2
Spacegroup: P43212
Unit cell:
a: 121.905Å b: 121.905Å c: 206.784Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.219 0.262
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

Fibronectin: a multidomain host adhesin targeted by bacterial fibronectin-binding proteins.
Henderson et al. (2011)
9 more

3 mentions without citation

Calcium binds to LipL32, a lipoprotein from pathogenic Leptospira, and modulates fibronectin binding.
Tung et al. (2010)
2 more