2wfx

X-ray diffraction
3.2Å resolution

Crystal structure of the complex between human hedgehog-interacting protein HIP and Sonic Hedgehog in the presence of calcium

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-178921 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Sonic hedgehog protein N-product Chain: A
Molecule details ›
Chain: A
Length: 152 amino acids
Theoretical weight: 17.37 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
UniProt:
  • Canonical: Q62226 (Residues: 40-191; Coverage: 37%)
Gene names: Hhg1, Shh
Sequence domains: Hedgehog amino-terminal signalling domain
Structure domains: Muramoyl-pentapeptide Carboxypeptidase; domain 2
Hedgehog-interacting protein Chain: B
Molecule details ›
Chain: B
Length: 457 amino acids
Theoretical weight: 50.97 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q96QV1 (Residues: 214-670; Coverage: 67%)
Gene names: HHIP, HIP, UNQ5825/PRO19644
Sequence domains:
Structure domains: Laminin

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P3221
Unit cell:
a: 89.15Å b: 89.15Å c: 171.429Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.245 0.24 0.3
Expression system: Homo sapiens