› 2wiq

X-ray diffraction
2Å resolution

Fluorescent protein KillerRed in the native state

Released:
DOI: 10.2210/pdb2wiq/pdb
PDB entry 2wiq coloured by chain, front view.
PDB entry 2wiq coloured by chain, side view.
PDB entry 2wiq coloured by chain, top view.
Source organism: Anthomedusae sp. DC-2005
Primary publication:
Structural basis for the phototoxicity of the fluorescent protein KillerRed.
Carpentier P, Violot S, Blanchoin L, Bourgeois D
FEBS Lett 583 2839-42 (2009)
PMID: 19646983

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174039 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
KillerRed Chains: A, B
Molecule details ›
Chains: A, B
Length: 257 amino acids
Theoretical weight: 28.73 KDa
Source organism: Anthomedusae sp. DC-2005
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q2TCH5 (Residues: 2-237; Coverage: 100%)
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

2 bound ligands:
Ligand SO4 3 x SO4
Ligand NA 2 x NA
1 modified residue:
Ligand CRQ 2 x CRQ

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P212121
Unit cell:
a: 71.49Å b: 73.67Å c: 80.26Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.171 0.236
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

19 review citations

Phototoxicity in live fluorescence microscopy, and how to avoid it.
Icha et al. (2017)
18 more

2 mentions without citation

Optogenetic control of ROS production.
Wojtovich et al. (2014)
1 more