› 2wjr

X-ray diffraction
1.8Å resolution

NanC porin structure in rhombohedral crystal form.

Released:
DOI: 10.2210/pdb2wjr/pdb
PDB entry 2wjr coloured by chain, front view.
PDB entry 2wjr coloured by chain, side view.
PDB entry 2wjr coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
NanC crystal structure, a model for outer-membrane channels of the acidic sugar-specific KdgM porin family.
Wirth C, Condemine G, Boiteux C, Bernèche S, Schirmer T, Peneff CM
J Mol Biol 394 718-31 (2009)
PMID: 19796645

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159676 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-acetylneuraminic acid outer membrane channel protein NanC Chain: A
Molecule details ›
Chain: A
Length: 214 amino acids
Theoretical weight: 25.39 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P69856 (Residues: 25-238; Coverage: 100%)
Gene names: JW5778, b4311, nanC, yjhA
Sequence domains: Oligogalacturonate-specific porin protein (KdgM)
Structure domains: monomeric porin ompg

Ligands and Environments

3 bound ligands:
Ligand EPE 2 x EPE
Ligand PO4 1 x PO4
Ligand OCT 4 x OCT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: R3
Unit cell:
a: 74.901Å b: 74.901Å c: 126.41Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.195 0.194 0.23
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Microbe-host interactions: structure and role of Gram-negative bacterial porins.
Galdiero et al. (2012)
3 more

10 mentions without citation

The structural biology of β-barrel membrane proteins: a summary of recent reports.
Fairman et al. (2011)
9 more