› 2wmf

X-ray diffraction
1.5Å resolution

Crystal structure of the catalytic module of a family 98 glycoside hydrolase from Streptococcus pneumoniae TIGR4 (Sp4GH98) in its native form.

Released:
DOI: 10.2210/pdb2wmf/pdb
PDB entry 2wmf coloured by chain, front view.
PDB entry 2wmf coloured by chain, side view.
PDB entry 2wmf coloured by chain, top view.
Source organism: Streptococcus pneumoniae TIGR4
Primary publication:
Differential recognition and hydrolysis of host carbohydrate antigens by Streptococcus pneumoniae family 98 glycoside hydrolases.
Higgins MA, Whitworth GE, El Warry N, Randriantsoa M, Samain E, Burke RD, Vocadlo DJ, Boraston AB
J Biol Chem 284 26161-73 (2009)
PMID: 19608744

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101490 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
F5/8 type C domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 581 amino acids
Theoretical weight: 66.74 KDa
Source organism: Streptococcus pneumoniae TIGR4
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H2US34 (Residues: 29-589; Coverage: 54%)
Gene name: SP_2159
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P212121
Unit cell:
a: 50.84Å b: 90.49Å c: 116.78Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.126 0.124 0.16
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Mucin glycan foraging in the human gut microbiome.
Tailford et al. (2015)
5 more

2 mentions without citation

Differential recognition and hydrolysis of host carbohydrate antigens by Streptococcus pneumoniae family 98 glycoside hydrolases.
Higgins et al. (2009)
1 more