› 2woo

X-ray diffraction
3.01Å resolution

Nucleotide-free form of S. pombe Get3

Released:
DOI: 10.2210/pdb2woo/pdb
PDB entry 2woo coloured by chain, front view.
PDB entry 2woo coloured by chain, side view.
PDB entry 2woo coloured by chain, top view.
Source organism: Schizosaccharomyces pombe
Primary publication:
The structural basis of tail-anchored membrane protein recognition by Get3.
Mateja A, Szlachcic A, Downing ME, Dobosz M, Mariappan M, Hegde RS, Keenan RJ
Nature 461 361-6 (2009)
PMID: 19675567

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-192859 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATPase get3 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 329 amino acids
Theoretical weight: 36.58 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9P7F8 (Residues: 1-329; Coverage: 100%)
Gene names: SPAC1142.06, get3
Sequence domains: Anion-transporting ATPase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand ZN 3 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P212121
Unit cell:
a: 73.795Å b: 92.922Å c: 286.468Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.237 0.288
Expression system: Escherichia coli

Quick links

Citations

33 review citations

Interactions between nuclei and the cytoskeleton are mediated by SUN-KASH nuclear-envelope bridges.
Starr et al. (2010)
32 more

8 mentions without citation

Tail-anchored membrane protein insertion into the endoplasmic reticulum.
Hegde et al. (2011)
7 more