2wpv

X-ray diffraction
1.99Å resolution

Crystal structure of S. cerevisiae Get4-Get5 complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-171300 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Golgi to ER traffic protein 4 Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 312 amino acids
Theoretical weight: 36.32 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q12125 (Residues: 1-312; Coverage: 100%)
Gene names: GET4, O3580, YOR164C
Sequence domains: Golgi to ER traffic protein 4
Structure domains: Tetratricopeptide repeat domain
Ubiquitin-like protein MDY2 Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 59 amino acids
Theoretical weight: 6.63 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q12285 (Residues: 1-59; Coverage: 28%)
Gene names: GET5, MDY2, TMA24, YOL111C
Sequence domains: Binding domain to Get4 on Get5, Golgi to ER traffic protein
Structure domains: Single helix bin

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P21
Unit cell:
a: 48.275Å b: 118.77Å c: 168.377Å
α: 90° β: 95.17° γ: 90°
R-values:
R R work R free
0.175 0.175 0.206
Expression system: Escherichia coli BL21(DE3)