› 2wpz

X-ray diffraction
1.25Å resolution

GCN4 leucine zipper mutant with two VxxNxxx motifs coordinating chloride

Released:
DOI: 10.2210/pdb2wpz/pdb
PDB entry 2wpz coloured by chain, front view.
PDB entry 2wpz coloured by chain, side view.
PDB entry 2wpz coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
A coiled-coil motif that sequesters ions to the hydrophobic core.
Hartmann MD, Ridderbusch O, Zeth K, Albrecht R, Testa O, Woolfson DN, Sauer G, Dunin-Horkawicz S, Lupas AN, Alvarez BH
Proc Natl Acad Sci U S A 106 16950-5 (2009)
PMID: 19805097

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136531 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
General control transcription factor GCN4 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 34 amino acids
Theoretical weight: 4.02 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
UniProt:
  • Canonical: P03069 (Residues: 249-281; Coverage: 12%)
Gene names: AAS101, AAS3, ARG9, GCN4, YEL009C
Sequence domains: Basic region leucine zipper

Ligands and Environments

1 bound ligand:
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P1
Unit cell:
a: 25.38Å b: 25.44Å c: 33.7Å
α: 83.98° β: 84.86° γ: 78.55°
R-values:
R R work R free
0.181 0.178 0.223
Expression system: Not provided

Quick links

Citations

14 review citations

Type V secretion: mechanism(s) of autotransport through the bacterial outer membrane.
Leo et al. (2012)
13 more

3 mentions without citation

A coiled-coil motif that sequesters ions to the hydrophobic core.
Hartmann et al. (2009)
2 more