› 2wq4

X-ray diffraction
1.42Å resolution

N-terminal domain of BC2L-C Lectin from Burkholderia cenocepacia

Released:
DOI: 10.2210/pdb2wq4/pdb
PDB entry 2wq4 coloured by chain, front view.
PDB entry 2wq4 coloured by chain, side view.
PDB entry 2wq4 coloured by chain, top view.
Source organism: Burkholderia cenocepacia J2315
Primary publication:
A TNF-like trimeric lectin domain from Burkholderia cenocepacia with specificity for fucosylated human histo-blood group antigens.
Sulák O, Cioci G, Delia M, Lahmann M, Varrot A, Imberty A, Wimmerová M
Structure 18 59-72 (2010)
PMID: 20152153

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lectin Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 156 amino acids
Theoretical weight: 15.96 KDa
Source organism: Burkholderia cenocepacia J2315
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B4EH86 (Residues: 1-156; Coverage: 57%)
Gene name: BCAM0185
Sequence domains: Bc2l-C, N-terminal domain
Structure domains: Jelly Rolls

Ligands and Environments

2 bound ligands:
Ligand SFU 3 x SFU
Ligand BR 1 x BR
1 modified residue:
Ligand MSE 9 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 43.1Å b: 76.73Å c: 103.26Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.133 0.131 0.166
Expression system: Escherichia coli BL21(DE3)

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Citations

5 review citations

Glycan microarrays for decoding the glycome.
Rillahan et al. (2011)
4 more

10 mentions without citation

Glycoscience@Synchrotron: Synchrotron radiation applied to structural glycoscience.
Pérez et al. (2017)
9 more