2wqt

X-ray diffraction
2.8Å resolution

Dodecahedral assembly of MhpD

Released:

Function and Biology Details

Reaction catalysed:
(S)-4-hydroxy-2-oxopentanoate = (2Z)-2-hydroxypenta-2,4-dienoate + H(2)O
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo 60-mer (preferred)
PDBe Complex ID:
PDB-CPX-160185 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-keto-4-pentenoate hydratase Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T
Length: 270 amino acids
Theoretical weight: 29.03 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P77608 (Residues: 1-269; Coverage: 100%)
Gene names: JW0341, b0350, mhpD
Sequence domains: Fumarylacetoacetate (FAA) hydrolase family
Structure domains: Fumarylacetoacetase-like, C-terminal domain

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: R3
Unit cell:
a: 207.35Å b: 207.35Å c: 545.471Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.223 0.222 0.234
Expression system: Escherichia coli BL21