› 2wqw

X-ray diffraction
2.24Å resolution

DOUBLE-DISULFIDE CROSS-LINKED CRYSTAL DIMER of the Listeria monocytogenes InlB internalin domain

Released:
DOI: 10.2210/pdb2wqw/pdb
PDB entry 2wqw coloured by chain, front view.
PDB entry 2wqw coloured by chain, side view.
PDB entry 2wqw coloured by chain, top view.
Source organism: Listeria monocytogenes EGD-e
Primary publication:
Ligand-mediated dimerization of the Met receptor tyrosine kinase by the bacterial invasion protein InlB.
Ferraris DM, Gherardi E, Di Y, Heinz DW, Niemann HH
J Mol Biol 395 522-32 (2010)
PMID: 19900460

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-144110 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Internalin B Chains: A, B
Molecule details ›
Chains: A, B
Length: 286 amino acids
Theoretical weight: 32.03 KDa
Source organism: Listeria monocytogenes EGD-e
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0DQD2 (Residues: 36-321; Coverage: 48%)
Gene names: inlB, lmo0434
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PEG 9 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: R32
Unit cell:
a: 186.99Å b: 186.99Å c: 115.071Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.201 0.199 0.239
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Targeting MET in cancer: rationale and progress.
Gherardi et al. (2012)
8 more