› 2wsi

X-ray diffraction
1.9Å resolution

Crystal structure of yeast FAD synthetase (Fad1) in complex with FAD

Released:
DOI: 10.2210/pdb2wsi/pdb
PDB entry 2wsi coloured by chain, front view.
PDB entry 2wsi coloured by chain, side view.
PDB entry 2wsi coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Crystal structure of yeast FAD synthetase (Fad1) in complex with FAD.
Leulliot N, Blondeau K, Keller J, Ulryck N, Quevillon-Cheruel S, van Tilbeurgh H
J Mol Biol 398 641-6 (2010)
PMID: 20359485

Function and Biology Details

Reaction catalysed: 
ATP + FMN = diphosphate + FAD
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153766 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Flavin adenine dinucleotide synthase Chain: A
Molecule details ›
Chain: A
Length: 306 amino acids
Theoretical weight: 35.58 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P38913 (Residues: 1-306; Coverage: 100%)
Gene names: D2702, FAD1, YDL045C
Sequence domains: Phosphoadenosine phosphosulfate reductase family
Structure domains: HUPs

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2
Unit cell:
a: 101.871Å b: 36.861Å c: 79.231Å
α: 90° β: 92.35° γ: 90°
R-values:
R R work R free
0.177 0.174 0.231
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Genetic control of biosynthesis and transport of riboflavin and flavin nucleotides and construction of robust biotechnological producers.
Abbas et al. (2011)
5 more

5 mentions without citation

Maturation of the respiratory complex II flavoprotein.
Sharma et al. (2019)
4 more